December 17, 2012 Monday - updated 13:00 PM PT

The Tip of Biotechnology Iceberg

new york, United States ( - December 17, 2012) Thanks to technology advance, humans are stepping closer to the truth and ideal statues if we ignore the still large number of unknowns. Here the author from Creative Biomart listed the following biotechnologies or biology-related method inventories that assist diagnostic and pharmaceutical research, biopharmaceutical drug development, clinical application and so on:

1. ELISA Kits
Virtually all microbial species have at least one antigen that is unique. These antigens can be purified and used to generate specific monoclonal antibodies. Both the antibodies and the purified antigens themselves provide effective and highly specific diagnostic tools. ELISA (enzyme-linked immunosorbent assay) now is a standard immunological technique used to detect the presence of an antigen or antibody in a sample, such as the newly testing method for HIV at home. In addition, it also is widely used in other industries, such as food allergen or toxicology in drug screening.

2.Protein Expression
Protein expression is a subcomponent of gene expression. It consists of the stages after DNA has been transcribed to messenger RNA (mRNA). The mRNA is then translated into polypeptide chains, which are ultimately folded into proteins. Protein expression is commonly used by proteomics researchers to denote the measurement of the presence and abundance of one or more proteins in a particular cell or tissue. Protein expression systems are very widely used in the life sciences, biotechnology and medicine. Molecular biology research uses numerous proteins and enzymes many of which are from expression systems; particularly DNA polymerase for PCR, reverse transcriptase for RNA analysis and restriction endonucleases for cloning. There are also significant medical applications for expression systems, notably the production of human insulin to treat diabetes. Protein expression systems are used to produce certain proteins in biotechnology and industry, and more recently to produce sets (combinatorial series) of protein that are screened for drug discovery purpose.

3. Protein Microarray
Similar to DNA microarrays, protein microarray (also known as protein chip) is another strategy for the large-scale study of proteins. It is a high-throughput method used to track the interactions and activities of proteins and to determine their functions in a parallel way. It may consist of a support surface such as a glass slide, nitrocellulose membrane, bead, or microtitre plate, to which an array of capture proteins is bound. Various types of probe molecules may be added to the array and assayed for ability to bind or react with the protein. Typically the probe molecules are labeled with a fluorescent dye, so that when the probe binds to the protein it results in a fluorescent signal that can be read by a laser scanner. This technology can complement other techniques, such as mass spectrometry and yeast two-hybrid assays. Protein microarrays are rapid, automated, economical, and highly sensitive, consuming small quantities of samples and reagents. It was relatively easy to develop since it is based on the previously-developed DNA microarray technology.

4. cDNA clone
In genetics, complementary DNA (cDNA) is DNA synthesized from a messenger RNA (mRNA) template in a reaction catalyzed by the enzymes reverse transcriptase and DNA polymerase. cDNA is often used to clone eukaryotic genes in prokaryotes. When scientists want to express a specific protein in a cell that does not normally express that protein (i.e,heterologous expression), they will transfer the cDNA that codes for the protein to the recipient cell. cDNA is also produced by retroviruses (such as HIV-1, HIV-2, Simian Immunodeficiency Virus, etc.) which is integrated into its host's genome where it creates a provirus.

Source: offered by Creative Biomart, a leading supplier that provides ELISA Kits, protein expression, PEGylation service, custom cDNA clone, protein microarray and other customized services. Creative Biomart has excellent R&D teams as well as rich experience; it provides the largest number of cDNA clones in multiple sets of vector systems with various features, including more than two hundred thousand cNDA clones; it has a long history in ELISA kits available in a range of formats including colorimetric, fluorescence, and chemiluminescence-based kits for various types of Analyte etc. With devotion and creation, in peer industry, Creative Biomart has gained good reputation with technical and price advantage.

Media Contact:

Serena lara
creative biomart
office worker
45-16 Ramsey Road, Shirley, NY
new york, USA

Phone: 1-516-669-8109
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